Protocols: New Tips & Tricks miniseries on maintaining and differentiating iPSCs

Growing stem cells and differentiating them into neuronal cells is difficult work. This miniseries has the tips and tricks I learned over the years for some help along the ways.

If you are only just starting up, culturing and differentiating stem cells is not a side project, if you want to do it well. It requires long term commitment (single differentiation rounds can easily go for many month) and permanent attention (like daily media changes of your stem cell cultures). Prepare yourself and your schedule as good as possible for it!

1st up: maintaining happy iPSC colonies

Next week: What to keep and what to throw out: identifying good colonies

Still coming:

  • Low-stress passaging of iPSC colonies
  • Too many choices: culture vessel options for differentiation
  • Too many choices: seeding density for differentiation
  • Too many choices: extracellular matrix coating for differentiation
  • Growth factor handling

If you are interested in differentiating stem cells into different neural cell types, have a look at my recent review about Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research.(Engel et al. 2016)

Engel, M. et al. 2016. Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research. Cellular and Molecular Life Sciences 73(19), pp. 3693–3709. Available at: http://dx.doi.org/10.1007/s00018-016-2265-3.
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